Human Reference Protein Interactome Project

One of the long-term goals at the Center for Cancer Systems Biology (CCSB) is to generate a first reference map of the human protein-protein interactome network. To reach this target, we are identifying binary protein-protein interactions (PPIs) by systematically interrogating all pairwise combinations of predicted human protein-coding genes using proteome-scale technologies. Our approach to map high-quality PPIs is based on using yeast two-hybrid (Y2H) as the primary screening method followed by validation of subsets of PPIs in multiple orthogonal assays for binary PPI detection.

All individual datasets are described in further details here and are freely available to the research community through the search engine or via downloadPreliminary data from ongoing projects are also described and available for search and download (email required).

Announcements and News

April 2018 release


We are pleased to annouce the release of our latest protein-protein interaction dataset, which contains more than 65,000 unique interactions.

10,000 New PPIs Released on HuRI Web Portal


For the first time, we have released PPIs identified from screening a space of 18k x 18k genes with a Y2H assay version that uses a C-terminal fusion of the Gal4 activation domain to our ORFs. The total number of human PPIs identified in systematic screens at CCSB now surpasses the 60,000.

Transition to GENCODE


We have fully transitioned to providing our interaction data using gene, transcript and protein identifiers as well as genome annotation information as provided by GENCODE.