The Center for Cancer Systems Biology at Dana-Farber Cancer Institute is generating the first complete reference map of the human protein-protein interactome network. All pairwise combinations of human protein-coding genes are systematically being interrogated to identifying which are involved in binary protein-protein interactions.
Pairwise combinations of human protein-coding genes are tested systematically using high throughput yeast two-hybrid screens to detect protein-protein interactions. The quality of these interactions are further validated in multiple orthogonal assays. Currently 75309 PPIs involving 11999 proteins have been identified using this framework.
In addition to systematically identifying PPIs experimentally, HuRI also includes PPIs of comparable high quality extracted from literature. This subset of literature-curated PPIs currently comprises 12589 PPIs.