HuRI
Proteins
11999
Interactions
74820

Mission

The Center for Cancer Systems Biology at Dana-Farber Cancer Institute is to generating the first complete reference map of the human protein-protein interactome network by systematically interrogating all pairwise combinations of human protein-coding genes to identifying which are involved in binary protein-protein interactions with eachother.

Announcements and News

2018 New Publication: “Protein Interactomics by Two-Hybrid Methods.”

2018-06-01

We are thrilled to present our publication in Methods in Molecular Biology where we discuss the methods developed for measuring different types of molecular interactions with an emphasis on direct protein-protein interactions, critical issues for generating high-quality interactome datasets, and the insights into biological networks and human diseases that current interaction mapping efforts provide.

 

https://www.ncbi.nlm.nih.gov/pubmed/?term=29855947

March 2018 New Study Published

2018-03-30

“Network Analysis of UBE3A/E6AP-Associated Proteins Provides Connections to Several Distinct Cellular Processes.”

We are excited to share our study published in Journal of Molecular Biology. This study contributes to a deeper understanding of the cellular functions of UBE3A and its potential role in pathways that may be affected in Angelman syndrome, UBE3A-associated autism spectrum disorders, and human papillomavirus-associated cancers by integrating UBE3A-specific interaction measurements with proteome-scale binary and co-complex interactome data.

https://www.ncbi.nlm.nih.gov/pubmed/?term=29426014

Feb. 2018: “How many human proteoforms are there?”

2018-02-14

Our study in Nature Chemical Biology asks the question, "How many distinct primary structures of proteins (proteoforms) are created from the 20,300 human genes?" It also explores prospects for improving measurements to better regularize protein-level biology and efficiently associate PTMs to function and phenotype.

https://www.ncbi.nlm.nih.gov/pubmed/?term=29443976    

Aug. 2017 New Prediction Method Publication

2017-08-28

“Domain-based prediction of the human isoform interactome provides insights into the functional impact of alternative splicing.”

In our new publication in PLOS Computational Biology, we present a domain-based method to predict the isoform interactome from the reference interactome. Our prediction framework is of high-quality when assessed by experimental data. Our prediction method complements experimental efforts, and demonstrates that integrating structural domain information with interactomes provides insights into the functional impact of alternative splicing.

 https://www.ncbi.nlm.nih.gov/pubmed/?term=28846689

May 2017 Newly Published in Trends Biochem Sci.: Proteome-Scale Human Interactomics.

2017-05-01

Our publication in Trends in Biochemical Sciences demonstrates knowledge gained from the recent publication of four human protein-protein interaction maps and complementary studies. It provides fresh insights into the opportunities and challenges when analyzing systematically generated interactome data and defines a clear roadmap towards the generation of a first human reference interactome map.

https://www.ncbi.nlm.nih.gov/pubmed/?term=28284537    

 

10,000 New PPIs Released on HuRI Web Portal

2017-07-21

For the first time, we have released PPIs identified from screening a space of 18k x 18k genes with a Y2H assay version that uses a C-terminal fusion of the Gal4 activation domain to our ORFs. The total number of human PPIs identified in systematic screens at CCSB now surpasses the 60,000.

Transition to GENCODE

2017-07-21

We have fully transitioned to providing our interaction data using gene, transcript and protein identifiers as well as genome annotation information as provided by GENCODE.

HuRI portal website is officially launched.

2017-01-08

HuRI portal website is officially launched.
All protein interaction data identified in systematic screens at CCSB published and unpublished is made publicly available under one access point for search and download.

Method

Pairwise combinations of human protein-coding genes are tested systematically using high throughp put yeast two-hybrid screens to detect protein-protein interactions. The quality of these interactions are further validated in multiple orthogonal assays. Currently 74820 PPIs involving 11999 proteins have been identified using this framework.

In addition to systematically identifying PPIs experimentally, HuRI also includes PPIs of comparable high quality extracted from literature. This subset of literature-curated PPIs is currently comprises 14595 PPIs involving xxx proteins.