The Center for Cancer Systems Biology at Dana-Farber Cancer Institute is to generating the first complete reference map of the human protein-protein interactome network by systematically interrogating all pairwise combinations of human protein-coding genes to identifying which are involved in binary protein-protein interactions with eachother.
Pairwise combinations of human protein-coding genes are tested systematically using high throughp put yeast two-hybrid screens to detect protein-protein interactions. The quality of these interactions are further validated in multiple orthogonal assays. Currently 74820 PPIs involving 11999 proteins have been identified using this framework.
In addition to systematically identifying PPIs experimentally, HuRI also includes PPIs of comparable high quality extracted from literature. This subset of literature-curated PPIs is currently comprises 14595 PPIs involving xxx proteins.